Mitchell M L
Clin Chem. 1976 Nov;22(11):1912-4.
I describe an improved procedure for estimating thyroxine in dried blood on 0.32-cm filter paper discs, by radioimmunoassay. A weak anion-exchange resin is used to separate free and antibody-bound radiothyroxine and, by measuring the resin-bound 125I-labeled thyroxine rather than the supernatant radioactivity, a tedious and cumbersome step is eliminated. The unique properties of the resin permit considerable latitude in the experimental conditions, in contrast with charcoal, without compromising sensitivity or precision. There is excellent correlation between thyroxine values obtained from discs and those of the corresponding plasmas. Application of this procedure for large-scale screening of newborns has proved to be highly successful in identifying thyroid insufficiency in several babies before clinical signs were apparent.
我描述了一种通过放射免疫分析法在0.32厘米滤纸圆盘上测定干血中甲状腺素的改进方法。使用一种弱阴离子交换树脂来分离游离的和与抗体结合的放射性甲状腺素，通过测量树脂结合的125I标记甲状腺素，而不是上清液的放射性，省去了一个繁琐且麻烦的步骤。与活性炭相比，该树脂的独特性质允许在实验条件上有相当大的灵活性，而不影响灵敏度或精密度。从圆盘获得的甲状腺素值与相应血浆的甲状腺素值之间具有极好的相关性。事实证明，将此方法应用于新生儿的大规模筛查，在数名婴儿出现临床症状之前识别出甲状腺功能不全方面非常成功。
