Costello P B, Green F A, Jung C Y
Arthritis Rheum. 1982 Jan;25(1):32-7. doi: 10.1002/art.1780250105.
Salicylate (SA) binding to albumin in purified human serum albumin (HSA) solutions was found to be predictably dependent on the concentrations of albumin and total SA. The influence of the presence of other ligands on SA binding in HSA solutions was measured and, in the case of bound fatty acids, free fractions of SA were increased by up to 100%. In sharp contrast, the free fraction of SA present in sera from patients with arthritis who were on long-term aspirin (ASA) therapy was roughly twice that found in HSA solutions. A correlation between serum concentrations of free SA and albumin was evident in sera from one group of children with juvenile rheumatoid arthritis (r = -0.75) but was not seen in sera from the other patients. This would indicate that in vivo, the albumin level is not the sole, or, in some patients, even the dominant determinant of the free fraction of SA. Additional factors such as fatty acids and other hydrophobic ligands, both endogenous and exogenous, should be studied further, especially in adults.
在纯化的人血清白蛋白（HSA）溶液中，发现水杨酸盐（SA）与白蛋白的结合可预测地取决于白蛋白和总SA的浓度。测量了其他配体的存在对HSA溶液中SA结合的影响，就结合脂肪酸的情况而言，SA的游离部分增加了高达100%。形成鲜明对比的是，长期服用阿司匹林（ASA）治疗的关节炎患者血清中SA的游离部分大约是HSA溶液中游离部分的两倍。在一组幼年类风湿性关节炎儿童的血清中，游离SA的血清浓度与白蛋白之间存在明显相关性（r = -0.75），但在其他患者的血清中未观察到这种相关性。这表明在体内，白蛋白水平不是SA游离部分的唯一决定因素，在某些患者中甚至不是主要决定因素。脂肪酸和其他内源性和外源性疏水配体等其他因素应进一步研究，尤其是在成年人中。
