缺氧对体外培养的人皮肤、肺和肌腱细胞的影响。
The effects of hypoxia on human skin, lung and tendon cells in vitro.
作者信息
Webster D F, Burry H C
出版信息
Br J Exp Pathol. 1982 Feb;63(1):50-5.
Abstract
Fibroblasts derived from human foetal tendon, skin and lung were cultured in media containing dissolved oxygen at concentrations of 20%, 10% and 2.7%. Total protein and collagen synthesis were reduced at 2.7% oxygen, tendon-derived cells proving significantly less sensitive than lung and skin fibroblasts. The effects of hypoxia were more marked in low-density cultures than in confluent cultures. Proliferation rates varied greatly between the 3 cell lines but were not affected by hypoxia. The implications of these results with respect to tendon degeneration and rupture are discussed.
摘要
从人胎儿肌腱、皮肤和肺中提取的成纤维细胞在含有浓度分别为20%、10%和2.7%溶解氧的培养基中培养。在2.7%的氧气浓度下，总蛋白和胶原蛋白合成减少，肌腱来源的细胞显示出比肺和皮肤成纤维细胞明显更低的敏感性。低氧对低密度培养物的影响比对汇合培养物的影响更显著。这三种细胞系的增殖率差异很大，但不受低氧影响。本文讨论了这些结果对肌腱退变和断裂的意义。
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