Jones W T, Broadhurst R B, Gurnsey M P
Biochim Biophys Acta. 1982 Mar 4;701(3):382-8. doi: 10.1016/0167-4838(82)90241-2.
Electrophoresis in 71/2% polyacrylamide gel separates bovine salivary proteins into 12 bands, and is used as the first step, followed by isoelectrofocusing or electrophoresis in polyacrylamide (15%) gels containing sodium dodecyl sulphate (SDS) or immunoelectrophoresis in two-dimensional separations. Molecular weights of native proteins are determined by electrophoresis on gels of polyacrylamide concentration 10 to 20%. Using these methods, information on native and subunit molecular weights, isoelectric pH values and immunological relationships for bovine salivary proteins has been determined. Enzymic functions for six of the proteins were established by histological methods.
在7.5%的聚丙烯酰胺凝胶中进行电泳可将牛唾液蛋白分离成12条带，此为第一步，随后进行等电聚焦或在含十二烷基硫酸钠（SDS）的15%聚丙烯酰胺凝胶中电泳，或在二维分离中进行免疫电泳。天然蛋白质的分子量通过在浓度为10%至20%的聚丙烯酰胺凝胶上进行电泳来测定。使用这些方法，已确定了牛唾液蛋白的天然和亚基分子量、等电pH值及免疫关系信息。其中六种蛋白质的酶功能通过组织学方法得以确定。
