Fliesler S J, Schroepfer G J
Biochim Biophys Acta. 1982 Apr 15;711(1):138-48. doi: 10.1016/0005-2760(82)90020-0.
The sterol composition of bovine retinal rod outer segment membranes and whole retinas was studied by detailed chromatographic analyses. Cholesterol represented at least 98% of the total 3 beta-monohydroxy sterols of rod outer segment membranes, accounting for 1.68 +/- 0.15% of the dry weight. Whole retinas contained 1.76 +/- 0.29% cholesterol by dry weight, representing at least 99% of the total 3 beta-monohydroxy sterols. Trace amounts of a component having the chromatographic properties of 5 alpha-cholestan-3 beta-ol were found in rod outer segment membranes and whole retinas. Very small amounts of a component having the chromatographic properties of 5 alpha-cholest-7-en-3 beta-ol were found in whole retinas, but not in rod outer segment membranes. The molar ratio of cholesterol to rhodopsin in bovine rod outer segment membranes was approximately 4.7. Cholesterol accounted for only 5-7 mol% of total rod outer segment membrane lipids.
通过详细的色谱分析研究了牛视网膜杆状体外段膜和整个视网膜的甾醇组成。胆固醇占杆状体外段膜总3β-单羟基甾醇的至少98%，占干重的1.68±0.15%。整个视网膜按干重计含有1.76±0.29%的胆固醇，占总3β-单羟基甾醇的至少99%。在杆状体外段膜和整个视网膜中发现了痕量具有5α-胆甾烷-3β-醇色谱特性的成分。在整个视网膜中发现了极少量具有5α-胆甾-7-烯-3β-醇色谱特性的成分，但在杆状体外段膜中未发现。牛杆状体外段膜中胆固醇与视紫红质的摩尔比约为4.7。胆固醇仅占杆状体外段膜总脂质的5 - 7摩尔%。
