Cronholm T, Rudqvist U
Biochim Biophys Acta. 1982 Apr 15;711(1):159-65. doi: 10.1016/0005-2760(82)90022-4.
Steroid sulphates were infused intravenously in female rats, and metabolites were isolated from bile. Infused 3 beta-hydroxy-5 alpha-androstan-17-one 3-sulphate was excreted together with 5 alpha-androstane-3 beta,17 beta-diol disulphate, which formed a large part after ethanol administration. Results from infusions of the 3-sulphates of 5 alpha-[17 alpha-2H]androstane-2 beta,17 beta-diol and 3 beta-hydroxy-5 alpha-[2,2,4,4-2H4]androstan-17-one indicated that ethanol decreased the extent of transfer of the 17 alpha-deuterium and increased the reduction of 17-oxosteroid without affecting the oxidation of 17 beta-hydroxysteroid. Ethanol metabolism decreased the deuterium transfer from [17 alpha-2H]estradiol 3-sulphate to C-17 of 3 beta-hydroxy-5 alpha-androstan-17-one 3-sulphate. The results indicate that NADH from ethanol metabolism increased the concentration of oxidoreductase-NADH complex without affecting the corresponding complex with NAD+. The effects of ethanol on steroid reduction were dependent on the initial redox state of the enzyme-coenzyme complex. This redox state was modified by substrates for the enzyme, indicating slow dissociation of the complex. Thus, ethanol metabolism may interfere with the interactions between steroid oxidoreductions.
将甾体硫酸盐静脉注射到雌性大鼠体内，然后从胆汁中分离代谢物。静脉注射的3β-羟基-5α-雄甾烷-17-酮3-硫酸盐与5α-雄甾烷-3β,17β-二醇二硫酸盐一起排泄，在给予乙醇后，后者占了很大一部分。对5α-[17α-2H]雄甾烷-2β,17β-二醇和3β-羟基-5α-[2,2,4,4-2H4]雄甾烷-17-酮的3-硫酸盐进行静脉注射的结果表明，乙醇降低了17α-氘的转移程度，并增加了17-氧代甾体的还原，而不影响17β-羟基甾体的氧化。乙醇代谢降低了[17α-2H]雌二醇3-硫酸盐向3β-羟基-5α-雄甾烷-17-酮3-硫酸盐C-17位的氘转移。结果表明，乙醇代谢产生的NADH增加了氧化还原酶-NADH复合物的浓度，而不影响与NAD+形成的相应复合物。乙醇对甾体还原的影响取决于酶-辅酶复合物的初始氧化还原状态。这种氧化还原状态被该酶的底物所改变，表明复合物的解离缓慢。因此，乙醇代谢可能会干扰甾体氧化还原之间的相互作用。
