Prima V I, Lisitsa E G, Platonov O M
Biokhimiia. 1982 Jan;47(1):145-52.
The mode of hybridization of dRNA fraction from normal and regenerating rat liver with purified fractions of repeating and unique sequences of DNA was studied. In order to repress the reassociation of DNA the nucleic acids were annealed in a solution of 2 x SSC--80% formamide at 48 degrees. The differential hybridization curves suggest that the amount of transcripts with a high DNA sequence frequency increases, while the complexity of transcripts of unique DNA sequences decreases 3 hrs after partial hepatectomy. Simultaneously the amount of RNA transcribed from the unique and moderately repeating DNA fractions remains practically unchanged.
研究了来自正常和再生大鼠肝脏的dRNA组分与纯化的DNA重复序列和单一序列组分的杂交模式。为了抑制DNA的重新缔合，核酸在2×SSC - 80%甲酰胺溶液中于48℃退火。差异杂交曲线表明，部分肝切除术后3小时，具有高DNA序列频率的转录本数量增加，而单一DNA序列转录本的复杂性降低。同时，从单一和中度重复DNA组分转录的RNA量实际上保持不变。
