Platonov O M, Lisitsa E G
Biokhimiia. 1980 May;45(5):896-900.
Transcription of unique and repeating sequences of DNA at early stages of liver regeneration after partial hepatectomy was studied. The total nuclear dRNA (tdRNA) and its two fractions (dRNA63O and dRNA85O) from the control and regenerating liver were subjected to hybridization with the excess of DNA. One hour after partial hepatectomy the kinetics of tdRNA hybridization did not differ from the controls; 3 hrs after operation the rate of unique transcripts hybridization was decreased. The rate of dRNA63O hybridization was shown to be considerably decreased 3 hrs after partial hepatectomy, while the kinetics of hybridization of dRNA85O remained unchanged. It was assumed that the changes of dRNA hybridization during the first few hours after partial hepatectomy are due to a significant increase in the amount of copies of some unique transcripts.
研究了部分肝切除术后肝脏再生早期DNA独特序列和重复序列的转录情况。将对照肝脏和再生肝脏的总核dRNA（tdRNA）及其两个组分（dRNA63O和dRNA85O）与过量的DNA进行杂交。部分肝切除术后1小时，tdRNA杂交动力学与对照无差异；术后3小时，独特转录本的杂交速率降低。部分肝切除术后3小时，dRNA63O的杂交速率显著降低，而dRNA85O的杂交动力学保持不变。据推测，部分肝切除术后最初几个小时内dRNA杂交的变化是由于一些独特转录本的拷贝数显著增加所致。
