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E组链球菌在化学成分明确的培养基中的生长及抗原产生

Growth of group E streptococci and production of antigens in a chemically defined medium.

作者信息

Wessman G E

出版信息

Can J Microbiol. 1982 Jan;28(1):106-10. doi: 10.1139/m82-010.

DOI:10.1139/m82-010
PMID:7066761
Abstract

Group E streptococci were grown in a modification of a chemically defined medium (CDM) originally formulated for streptococci of group A. Ten of 12 strains studied produced greater or equal cultural densities and growth rates in CDM than in the complex medium (Todd-Hewitt broth). All strains produced similar amounts of group antigen in both media. Eight strains of demonstrable serotype produced comparable amounts of type antigen in both media. cells grown in CDM were less resistant to phagocytosis than those grown in the complex medium, but all strains produced antiphagocytic factor when cultured in either medium to which 10% porcine serum had been added.

摘要

E组链球菌在一种对最初为A组链球菌配制的化学成分确定培养基(CDM)进行改良后的培养基中生长。在所研究的12株菌株中,有10株在CDM中的培养密度和生长速率高于或等于在复合培养基(托德-休伊特肉汤)中的情况。所有菌株在两种培养基中产生的群抗原量相似。8株可鉴定血清型的菌株在两种培养基中产生的型抗原量相当。在CDM中生长的细胞比在复合培养基中生长的细胞对吞噬作用的抵抗力弱,但当在添加了10%猪血清的任何一种培养基中培养时,所有菌株都会产生抗吞噬因子。

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