流式细胞分选的蜕膜细胞核中的倍性和孕激素受体分布
Ploidy and progesterone-receptor distribution in flow-sorted deciduomal nuclei.
作者信息
McConnell K N, Sillar R G, Young B D, Green B
出版信息
Mol Cell Endocrinol. 1982 Jan;25(1):99-104. doi: 10.1016/0303-7207(82)90172-1.
PMID:7067927
Abstract
Nuclei from rat uterine deciduomata were analysed and sorted into different ploidy classes in a fluorescence-activated cell sorter. The highest proportion of 8 n and 16 n nuclei was observed on days 6-7 after decidualization. At this time the progesterone receptor content per unit DNA of the octaploid nuclei was less than half that of the diploid nuclei.
摘要
对大鼠子宫蜕膜瘤的细胞核进行分析，并在荧光激活细胞分选仪中分类为不同的倍体类别。在蜕膜化后第6 - 7天观察到8倍体和16倍体细胞核的比例最高。此时，八倍体细胞核每单位DNA的孕酮受体含量不到二倍体细胞核的一半。
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