Vatn M H, Tjora S, Arva P H, Serck-Hanssen A, Stromme J H
Gut. 1982 Mar;23(3):194-7. doi: 10.1136/gut.23.3.194.
Enzymes activities in homogenates of the large intestine were measured in biopsies from 11 tubular adenomas and six carcinomas and from the corresponding normal mucosa of each individual. A similar and significant increase was found in the total activities of glucose-6-phosphate dehydrogenase (G 6 PD), pyruvate kinase, and lactate dehydrogenase in adenomas and carcinomas as compared with the levels of normal mucosa. The lactate dehydrogenase isoenzyme patterns showed also a marked and similar change in the adenomas and carcinomas with a significant increase of lactate dehydrogenase M-monomers. Most of the adenomas showed slight to moderate dysplasia. The results indicate that the measurement of total G 6 PD, pyruvate kinase and lactate dehydrogenase activities for screening purposes has no predictive value in separating non-premalignant from premalignant adenomas. Moreover, it seems that the enzymatic characteristics of malignancy are present even in tubular adenomas. This is in agreement with the theory of a polyp-cancer sequence.
在11例管状腺瘤和6例癌以及每个个体相应的正常黏膜的活检组织中，测定了大肠匀浆中的酶活性。与正常黏膜水平相比，腺瘤和癌组织中葡萄糖-6-磷酸脱氢酶（G 6 PD）、丙酮酸激酶和乳酸脱氢酶的总活性有相似且显著的增加。乳酸脱氢酶同工酶谱在腺瘤和癌组织中也显示出明显且相似的变化，乳酸脱氢酶M-单体显著增加。大多数腺瘤表现为轻度至中度发育异常。结果表明，为筛查目的而测量总G 6 PD、丙酮酸激酶和乳酸脱氢酶活性，对于区分非癌前腺瘤和癌前腺瘤没有预测价值。此外，似乎即使在管状腺瘤中也存在恶性肿瘤的酶学特征。这与息肉-癌序列理论相符。
