Lesley J, Trowbridge I S
Immunogenetics. 1982 Mar;15(3):313-20. doi: 10.1007/BF00364339.
As described in the preceding paper, monoclonal antibodies have been raised by immunization of rats with mouse hematopoietic cells which detect a major cell-surface glycoprotein (Mr = 95 000) of mouse bone-marrow cells of the granulocytic series. While most of the monoclonal antibodies detect this molecule one bone-marrow and spleen cells of all mouse strains, two antibodies recognize alternative allelic forms of the molecule. One alloantigen is expressed in all the remaining inbred strains examined. The alloantigens are codominantly expressed on the cells of F1 mice. Backcrosses of DBA/2 and C57BL/6 with F1 mice (B6D2F1) confirmed that a single genetic locus is involved in the expression of the two antigenic forms and demonstrated linkage to Ly-m11 which has previously been mapped to mouse chromosome 2. These genetic mapping experiments and the biochemical properties of the glycoprotein suggested that it might be identical to a glycoprotein first identified on murine fibroblasts by Hughes and August and designated Pgp-1. This has been firmly established by exchange of monoclonal antibody reagents and sequential immunoprecipitations.
如前文所述，通过用小鼠造血细胞免疫大鼠产生了单克隆抗体，这些抗体可检测粒细胞系小鼠骨髓细胞的一种主要细胞表面糖蛋白（分子量 = 95000）。虽然大多数单克隆抗体能在所有小鼠品系的骨髓和脾细胞中检测到这种分子，但有两种抗体识别该分子的替代等位基因形式。一种同种异型抗原在所有检测的其余近交系中表达。这些同种异型抗原在F1小鼠的细胞上共显性表达。DBA/2和C57BL/6与F1小鼠（B6D2F1）的回交证实，单一基因座参与了两种抗原形式的表达，并证明与先前已定位到小鼠2号染色体的Ly-m11连锁。这些遗传定位实验和糖蛋白的生化特性表明，它可能与休斯和奥古斯特首先在鼠成纤维细胞上鉴定并命名为Pgp-1的糖蛋白相同。通过单克隆抗体制剂的交换和连续免疫沉淀已明确证实了这一点。
