Tada N, Kimura S, Hatzfeld A, Hämmerling U
Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
Immunogenetics. 1980;11(5):441-9. doi: 10.1007/BF01567813.
Spleen cells from an SJL mouse immunized with B10.S spleen cells were fused with the nonsecretor myeloma line NS.1. One established hybrid cell line continuously secreted antibody that recognized a new antigenic specificity, tentatively called "Ly-m11." This newly found antigen is detectable on nearly 100 percent of spleen and lymph-node cells, 70 percent of bone-marrow cells, and 20 percent of thymus cells by direct cytotoxicity assays, and on the cells derived from kidney and liver. Strains that are Ly-m11 (+) include C57BL/6, C57BL/10J, B10.S, C57BR/cdJ, C57L/J, and C57BL/KsJ. Other mouse strains so far tested are Ly-m11 (-). The strain distribution pattern distinguished Ly-m11 from any known murine lymphocyte alloantigens, but it follows the H-3 alpha haplotype which is defined by skin transplantation. Linkage tests of nine congenic strains of H-3 and/or H-13/alpha loci and five recombinant inbred lines including CXB, BXH, AKXL, SWXL, and BXD revealed no recombinations between H-3 and Ly-m11 loci on chromosome 2. This newly discovered Ly-m11 alloantigen could itself constitute a minor histocompatibility antigen detectable by serological means.
用B10.S脾细胞免疫的SJL小鼠的脾细胞与非分泌型骨髓瘤细胞系NS.1进行融合。建立的一个杂交细胞系持续分泌能识别一种新抗原特异性的抗体，暂称为“Ly-m11”。通过直接细胞毒性试验，在近100%的脾细胞和淋巴结细胞、70%的骨髓细胞以及20%的胸腺细胞上可检测到这种新发现的抗原，在源自肾脏和肝脏的细胞上也能检测到。Ly-m11(+)的品系包括C57BL/6、C57BL/10J、B10.S、C57BR/cdJ、C57L/J和C57BL/KsJ。到目前为止检测的其他小鼠品系均为Ly-m11(-)。这种品系分布模式使Ly-m11与任何已知的小鼠淋巴细胞同种异体抗原区分开来，但它遵循由皮肤移植所定义的H-3α单倍型。对9个H-3和/或H-13/α位点的同类系以及包括CXB、BXH、AKXL、SWXL和BXD在内的5个重组近交系进行连锁测试，结果显示2号染色体上的H-3和Ly-m11位点之间没有重组。这个新发现的Ly-m11同种异体抗原本身可能构成一种可用血清学方法检测的次要组织相容性抗原。
