Boner A, Zeligs B J, Bellanti J A
Infect Immun. 1982 Mar;35(3):921-8. doi: 10.1128/iai.35.3.921-928.1982.
Chemotactic response and responsiveness, that is, the ability to respond to various concentrations of the chemoattractant zymosan-activated serum was measured for cord and adult blood neutrophils. Chemotactic response of neutrophils from adult blood was two to seven times greater than that of neutrophils from cord blood. In addition, neutrophils from cord blood showed poor response to concentrations of 25% chemoattractant or less, compared to those from adults, which responded to concentrations of 10%. Further, it was found that approximately 30% of neutrophils in cord blood were the band form compared to only 9% in adult. Based upon this, a simple method was devised to assay mean migrating distance of various differentiational stages of neutrophils incorporating both distance and magnitude of their responses. The results of these assays showed that all differentiational stages of cord blood neutrophils have a mean migrating distance less than those of adults. In addition, the band form from both cord and adult blood had a mean migrating distance less than the polymorphonuclear form. Adherence studies revealed that all differentiational stages of neutrophils adhered in a similar manner, and no difference was detected between cells from cord and adult blood. Assays to test the ability of cord blood to produce chemotactic activity when activated revealed 50% less activity than that obtained from adult serum which further decreased with dilution of attractant. These data suggest that both the cellular and humoral systems involved in chemotactic responses are less in cord blood compared to adult blood.
对脐带血和成人血液中的中性粒细胞，测定了趋化反应和反应性，即对不同浓度的趋化剂酵母聚糖激活血清的反应能力。成人血液中性粒细胞的趋化反应比脐带血中性粒细胞的趋化反应大两到七倍。此外，与对10%浓度有反应的成人中性粒细胞相比，脐带血中性粒细胞对25%或更低浓度的趋化剂反应较差。此外，发现脐带血中约30%的中性粒细胞为杆状核，而成人仅为9%。基于此，设计了一种简单的方法来测定中性粒细胞不同分化阶段的平均迁移距离，该方法综合考虑了其反应的距离和强度。这些测定结果表明，脐带血中性粒细胞的所有分化阶段的平均迁移距离均小于成人。此外，脐带血和成人血液中的杆状核中性粒细胞的平均迁移距离均小于多形核中性粒细胞。黏附研究表明，中性粒细胞的所有分化阶段以相似的方式黏附，脐带血和成人血液中的细胞之间未检测到差异。测试脐带血激活时产生趋化活性能力的试验显示，其活性比成人血清低50%，且随着趋化剂稀释进一步降低。这些数据表明，与成人血液相比，脐带血中参与趋化反应的细胞和体液系统均较少。
