视黄酸5,6 - 环氧化酶。性质及生物学意义。

Retinoic acid 5,6-epoxidase. Properties and biological significance.

作者信息

Sietsema W K, DeLuca H F

出版信息

J Biol Chem. 1982 Apr 25;257(8):4265-70.

Abstract

A retinoic acid epoxidase is present in rat kidney homogenates. It is found in the particulate fraction, and is dependent on ATP, NADPH, and oxygen. It is stimulated by Fe2+ and inhibited by Mn2+, Zn2+, Cu2+, EDTA, and N,N'-diphenyl-para-phenylenediamine. Its properties are closest to those of microsomal lipid peroxidases previously studied. Epoxidase activity is not affected by the rat's vitamin A status, nor is it inducible by retinoic acid. In N,N'-diphenyl-para-phenylenediamine-feeding experiments, it was shown that blocking epoxidation in vivo does not inhibit the function of retinoic acid. It is concluded that retinoic acid epoxidation is not required for retinoic acid function.

摘要

大鼠肾脏匀浆中存在一种视黄酸环氧化酶。它存在于微粒体部分，依赖于ATP、NADPH和氧气。它受Fe2+刺激，受Mn2+、Zn2+、Cu2+、EDTA和N,N'-二苯基对苯二胺抑制。其性质与先前研究的微粒体脂质过氧化物酶最为接近。环氧化酶活性不受大鼠维生素A状态的影响，也不能被视黄酸诱导。在N,N'-二苯基对苯二胺喂养实验中，结果表明体内阻断环氧化并不抑制视黄酸的功能。得出的结论是，视黄酸发挥功能不需要视黄酸环氧化。