Chan A C, Pritchard E T, Choy P C
J Nutr. 1983 Apr;113(4):813-9. doi: 10.1093/jn/113.4.813.
This study was designed to evaluate the effects of dietary vitamin E and synthetic antioxidants on prostacyclin (PGI2) synthesis in isolated aorta segments and perfused hearts as well as thromboxane (TxA2) synthesis in thrombin-stimulated washed platelets. Weanling male New Zealand rabbits were fed a vitamin E-deficient basal diet or the basal diet supplemented with either all-rac-alpha-tocopherol acetate or propyl gallate or DPPD (N,N'-diphenyl-p-phenylenediamine). After 30 days on the diet, plasma tocopherol level, pyruvate kinase and liver microsomal NADPH oxidase were determined. DPPD but not propyl gallate prevented the development of myopathy. None of the synthetic antioxidants could substitute for vitamin E in decreasing enzymatic lipid peroxidation. PGI2 release by the aorta was lowered in vitamin E deficiency and was highest with DPPD supplementation. In the Langendorff perfused heart, however, PGI2 release was highest in the vitamin E-deficient group, possibly due to cardiomyopathy. TxA2 synthesis by washed platelets challenged with thrombin was independent of the antioxidant status of the animal. The data showed that dietary antioxidants selectively affect eicosanoid synthesis in different tissues.
本研究旨在评估膳食维生素E和合成抗氧化剂对离体主动脉段和灌注心脏中前列环素（PGI2）合成以及凝血酶刺激的洗涤血小板中血栓素（TxA2）合成的影响。将断乳雄性新西兰兔喂食维生素E缺乏的基础日粮，或补充了消旋-α-生育酚醋酸酯、没食子酸丙酯或N,N'-二苯基对苯二胺（DPPD）的基础日粮。在日粮喂养30天后，测定血浆生育酚水平、丙酮酸激酶和肝微粒体NADPH氧化酶。DPPD而非没食子酸丙酯可预防肌病的发生。在降低酶促脂质过氧化方面，没有一种合成抗氧化剂能够替代维生素E。维生素E缺乏时主动脉释放的PGI2降低，补充DPPD时最高。然而，在Langendorff灌注心脏中，PGI2释放量在维生素E缺乏组中最高，这可能是由于心肌病所致。凝血酶刺激的洗涤血小板合成TxA2与动物的抗氧化状态无关。数据表明，膳食抗氧化剂可选择性地影响不同组织中类花生酸的合成。
