Hilburn L R, Rai K S
J Hered. 1982 Jan-Feb;73(1):59-63. doi: 10.1093/oxfordjournals.jhered.a109576.
When male hybrids of Aedes aegypti females and A. mascarensis males were backcrossed to A. aegypti females, 32.8 percent of the male progeny exhibited abnormal sexual development, including failure of the terminalia to rotate, a split sternite of the eighth abdominal segment with partially duplicated telomeres, or feminization that gives rise to sterile intersexes. Observations made on three morphological marker loci and five isozyme loci with characteristic electromorphs in the two parental species suggested that when the sex-determining M locus is derived from A. mascarensis and the chromosome regions including s, LDH, and lDH2 on chromosome 2 and blt and 6PGD on chromosome 3 are homozygous for genes from A. aegypti, the frequency of abnormal sexual development is increased. An even greater percentage of males suffer aberrant development if recombination also occurs between the M and re locus of chromosome 1. The data suggest that genes on chromosome 2 control normal development of the male terminalia, genes on chromosome 3 control sexual differentiation, and the entire process is controlled by genes on chromosome 1 that are linked to, but not identical with, the M locus.
当埃及伊蚊雌蚊与马斯卡林伊蚊雄蚊的雄性杂交后代与埃及伊蚊雌蚊回交时，32.8%的雄性后代表现出异常的性发育，包括外生殖器无法旋转、第八腹节腹板裂开且端粒部分重复，或出现导致不育雌雄同体的雌性化现象。对两个亲本物种中具有特征性电泳形态的三个形态标记位点和五个同工酶位点的观察表明，当性别决定的M位点来自马斯卡林伊蚊，且2号染色体上包括s、LDH和lDH2以及3号染色体上的blt和6PGD的染色体区域对来自埃及伊蚊的基因呈纯合状态时，异常性发育的频率会增加。如果1号染色体的M和re位点之间也发生重组，那么会有更高比例的雄性出现发育异常。数据表明，2号染色体上的基因控制雄性外生殖器的正常发育，3号染色体上的基因控制性分化，而整个过程由1号染色体上与M位点连锁但不相同的基因控制。
