Hammar H, Halprin K
J Invest Dermatol. 1982 Apr;78(4):300-4. doi: 10.1111/1523-1747.ep12507369.
Adult pig skin was stripped of its horny layer by cellophane tape. At intervals over the next 4 days explant cultures were prepared from such areas. Growth displayed an initial rapid and secondary slower phase. Plating of explants immediately following the stripping gave rise to an increased rate of migration of cells from the explants during the first growth phase but a decreased second or proliferative growth phase. This was not present when plating was delayed 1 to 4 days after stripping. The growth fraction in cells of the stripped skin explant cultures was similar to that of unstripped controls. The diminished growth rate of the cells was due to prolongation of the mean cell cycle time. Thus, the hyperproliferative state induced in vivo after stripping did not maintain itself when transferred in vitro. This result is of importance concerning animal models for psoriasis and the influence a transfer to in vitro conditions may have on the hyperproliferative psoriatic epidermis.
用玻璃纸胶带剥去成年猪皮肤的角质层。在接下来的4天里，每隔一段时间就从这些部位制备外植体培养物。生长呈现出最初的快速期和随后的缓慢期。剥皮后立即接种外植体，在第一个生长阶段，外植体细胞的迁移速率增加，但在第二个或增殖生长阶段则降低。剥皮后1至4天延迟接种时则不存在这种情况。剥皮皮肤外植体培养物中细胞的生长分数与未剥皮对照相似。细胞生长速率的降低是由于平均细胞周期时间延长。因此，剥皮后在体内诱导的增殖状态在体外转移时不能维持自身。这一结果对于银屑病动物模型以及转移到体外条件对银屑病增生性表皮的影响具有重要意义。
