Berrutti L E, Singer A J, McClain S A
Department of Dermatology, Division of Dermatopathology, State University of New York, Stony Brook, NY, USA.
Acad Emerg Med. 2000 Dec;7(12):1349-53. doi: 10.1111/j.1553-2712.2000.tb00490.x.
The stratum corneum (SC) is the major barrier to topical absorption of medications. Skin tape stripping (TS) removes the stratum corneum, allowing more rapid absorption of drugs such as local anesthetics. Prior to evaluating TS in human clinical trials, this study was performed to evaluate its immediate and delayed histopathologic effects in swine.
This was a prospective, interventional, longitudinal, experimental animal study using two isoflurane-anesthetized young swine. Cellophane tape was applied to the skin of clipped swine flanks and peeled away 10, 15, 20, 25, or 30 times. Each level of tape stripping was performed three times in each pig. Full-thickness biopsies were taken at 30 minutes and two weeks later for blinded histopathologic evaluation by a dermatopathologist using randomly ordered hematoxylin-and-eosin-stained tissue sections and conventional light microscopy. The absolute thickness of the cornified layer was measured and compared with normal (unstripped) cornified layer.
Tape stripping up to 30 times produces thinning of the SC without detectable changes in the underlying epidermis and dermis at 30 minutes and 14 days post-stripping. The degree of thinning was proportional to the number of tape applications. Complete recovery of the cornified layer was noted at two weeks. There were no adverse effects, such as infection, scarring, or inflammatory cell infiltrates.
Under the conditions studied, TS not only appears safe, but appears to produce no long-term sequelae.
角质层（SC）是药物经皮吸收的主要屏障。皮肤胶带剥离（TS）可去除角质层，使局部麻醉药等药物吸收更快。在人体临床试验中评估TS之前，进行本研究以评估其对猪的即时和延迟组织病理学影响。
这是一项前瞻性、干预性、纵向实验动物研究，使用两只异氟烷麻醉的幼猪。将玻璃纸胶带贴在剃毛后的猪侧腹皮肤上，并剥离10、15、20、25或30次。每只猪对每个胶带剥离水平进行三次操作。在30分钟和两周后取全层活检组织，由皮肤病理学家使用随机排序的苏木精-伊红染色组织切片和传统光学显微镜进行盲法组织病理学评估。测量角质层的绝对厚度，并与正常（未剥离）角质层进行比较。
在胶带剥离30次以内，在剥离后30分钟和14天时，角质层变薄，而其下方的表皮和真皮未检测到变化。变薄程度与胶带粘贴次数成正比。两周时观察到角质层完全恢复。未出现感染、瘢痕形成或炎性细胞浸润等不良反应。
在所研究的条件下，TS不仅看起来安全，而且似乎不会产生长期后遗症。
