Asperger O, Kleber H P, Aurich H
Acta Biol Med Ger. 1978;37(2):191-8.
The qualitative and quantitative composition of cytochromes in intact cells of Acinetobacter calcoaceticus and in particle fractions obtained from cells following ultrasonic treatment by differential centrifugation were studied using spectrophotometric methods. Acinetobacter calcoaceticus contains cytochrome b, cytochrome o and low amounts of cytochrome d. Both the absolute content of cytochrome b and o and the relative composition do not essentially vary with the carbon source used (hexadecane, acetate, succinate, malate, yeast extract). Only bacterial cultivated on yeast extract show, under simultaneous decrease of the content of cytochrome o, an increased formation of cytochrome d. In Acinetobacter, cytochromes appear not to be immediately involved in n-alkane hydroxylation.
采用分光光度法研究了醋酸钙不动杆菌完整细胞以及经超声处理后通过差速离心从细胞中获得的颗粒组分中细胞色素的定性和定量组成。醋酸钙不动杆菌含有细胞色素b、细胞色素o和少量的细胞色素d。细胞色素b和o的绝对含量以及相对组成基本上不随所使用的碳源（十六烷、乙酸盐、琥珀酸盐、苹果酸盐、酵母提取物）而变化。只有在酵母提取物上培养的细菌在细胞色素o含量同时降低的情况下，细胞色素d的形成增加。在不动杆菌中，细胞色素似乎不直接参与正构烷烃的羟基化作用。
