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培养的鼠麻风杆菌中的细胞色素系统。

Cytochrome system in cultivated Mycobacterium lepraemurium.

作者信息

Ishaque M

出版信息

Cytobios. 1984;39(155-156):165-70.

PMID:6428810
Abstract

The respiratory pigments of cell suspensions of Mycobacterium lepraemurium cultivated on Ogawa egg-yolk medium were investigated spectrophotometrically. The results obtained showed that whole cell suspensions of both Kumato and Hawaiian strains contained flavins, cytochromes of the a2 and b type, as well as a CO-binding pigment similar to cytochrome o. The whole cell suspensions of M. lepraemurium did not show detectable quantities of c type cytochrome. However, cytochrome c was present in small amounts, and its presence became evident in the dithionite-reduced minus oxidized difference spectra of pyridine haemochromogens prepared from in vitro grown cells of M. lepraemurium.

摘要

用分光光度法研究了在小川蛋黄培养基上培养的鼠麻风分枝杆菌细胞悬液的呼吸色素。所得结果表明,熊本菌株和夏威夷菌株的全细胞悬液均含有黄素、a2和b型细胞色素,以及一种类似于细胞色素o的一氧化碳结合色素。鼠麻风分枝杆菌的全细胞悬液未检测到c型细胞色素。然而,细胞色素c含量较少,从体外培养的鼠麻风分枝杆菌细胞制备的吡啶血色原的连二亚硫酸盐还原减去氧化差光谱中,其存在变得明显。

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