Rostami A, Lisak R P, Blanchard N, Guerrero F, Zweiman B, Pleasure D
J Neurol Sci. 1982 Mar;53(3):433-41. doi: 10.1016/0022-510x(82)90241-6.
The cerebrospinal fluid (CSF) of guniea pigs with experimental allergic encephalomyelitis was examined for the presence of oligoclonal IgG using polyacrylamide gel electrophoresis. Oligoclonal IgG (greater than or equal to 2 bands) was seen in the CSF obtained from 3/4 animals with experimental allergic encephalomyelitis induced by myelin basic protein and 2/3 with spinal cord-induced disease. It was not seen in CSF of 3 non-sensitized, 4 adjuvant-sensitized and 7 liver-sensitized guinea pigs. Scanning of stained gels confirmed the oligoclonal pattern. The bands were found in the region of gels which bound [125I]Staphylococcal Protein A. The data demonstrate that a non-infectious inflammatory reaction within the central nervous system can result in an oligoclonal IgG pattern in the CSF.
运用聚丙烯酰胺凝胶电泳法，检测了患有实验性变应性脑脊髓炎的豚鼠脑脊液中寡克隆IgG的存在情况。在由髓鞘碱性蛋白诱发实验性变应性脑脊髓炎的4只动物中，有3只的脑脊液中可见寡克隆IgG（大于或等于2条带）；在由脊髓诱发疾病的动物中，3只中有2只的脑脊液中可见寡克隆IgG。在3只未致敏、4只佐剂致敏和7只肝脏致敏的豚鼠脑脊液中未见到寡克隆IgG。对染色凝胶进行扫描证实了寡克隆模式。这些条带出现在凝胶中与[125I]葡萄球菌蛋白A结合的区域。数据表明，中枢神经系统内的非感染性炎症反应可导致脑脊液中出现寡克隆IgG模式。
