Olsson T, Kristensson K, Leijon G, Link H
J Neurol Sci. 1982 Jun;54(3):359-75. doi: 10.1016/0022-510x(82)90200-3.
Chronic relapsing allergic encephalomyelitis (r-EAE) was induced in a local strain of guinea pigs. By the use of isoelectric focusing (IF) followed by antigen immunofixation and autoradiography, antibodies directed against central nervous system (CNS) myelin were detected in 21 of 23 sera sampled during the course of r-EAE. Previous absorption of the sera with CNS myelin reduced or abolished antibody activity on autoradiograms. One r-EAE guinea pig developed definite oligoclonal IgG bands in serum while in 7 r-EAE animals faint oligoclonal IgG bands were present. The mobility of oligoclonal IgG bands differed from the mobility of antimyelin antibody bands on autoradiograms. The significance of these findings has not been definitely elucidated but the antimyelin antibodies may possibly be involved in the pathogenesis of the disease while oligoclonal IgG bands may represent an epiphenomenon not pathogenetically related to r-EAE.
在一种本地豚鼠品系中诱发了慢性复发性变应性脑脊髓炎（r-EAE）。通过等电聚焦（IF），随后进行抗原免疫固定和放射自显影，在r-EAE病程中采集的23份血清中的21份中检测到了针对中枢神经系统（CNS）髓磷脂的抗体。先前用CNS髓磷脂对血清进行吸收可降低或消除放射自显影片上的抗体活性。一只r-EAE豚鼠血清中出现了明确的寡克隆IgG带，而在7只r-EAE动物中存在微弱的寡克隆IgG带。放射自显影片上寡克隆IgG带的迁移率与抗髓磷脂抗体带的迁移率不同。这些发现的意义尚未明确阐明，但抗髓磷脂抗体可能参与了该疾病的发病机制，而寡克隆IgG带可能代表一种与r-EAE发病机制无关的附带现象。
