Calvert C C, Klasing K C, Austic R E
J Nutr. 1982 Apr;112(4):627-35. doi: 10.1093/jn/112.4.627.
The role of food intake and branched-chain amino acid (BCAA) catabolism in the branched-chain amino acid antagonism was investigated. A diet containing crystalline amino acids as the sole source of amino acids was formulated to contain adequate levels of all required nutrients. The basal diet contained 0.60% isoleucine, 0.82% valine and 1.2% leucine. Increasing dietary leucine to 5.0% resulted in reduced food consumption, weight gain, and efficiency of food utilization. These effects were prevented by increasing dietary isoleucine and valine to 0.80 and 1.07%, respectively. When L-[1-14C]isoleucine or L[1-14C]valine were included in the diet, the amont of 14CO2 exhaled was increased within 24 hours of feeding the 5% leucine diet. The excretion of 14C was unaffected by leucine. It was determined by force feeding that approximately 70% of the reduced growth rate in chicks fed the leucine-supplemented diet ad libitum could be accounted for by reduced food intake. A portion of the growth depression may be due to increased BCAA catabolism, limiting the availability of valine and isoleucine for growth.
研究了食物摄入和支链氨基酸（BCAA）分解代谢在支链氨基酸拮抗作用中的作用。配制了一种以结晶氨基酸作为唯一氨基酸来源的日粮，使其含有所有必需营养素的适当水平。基础日粮含有0.60%的异亮氨酸、0.82%的缬氨酸和1.2%的亮氨酸。将日粮亮氨酸增加到5.0%会导致食物消耗、体重增加和食物利用率降低。分别将日粮异亮氨酸和缬氨酸增加到0.80%和1.07%可防止这些影响。当日粮中包含L-[1-14C]异亮氨酸或L-[1-14C]缬氨酸时，在饲喂5%亮氨酸日粮的24小时内呼出的14CO2量增加。14C的排泄不受亮氨酸的影响。通过强制喂食确定，随意采食亮氨酸补充日粮雏鸡生长率降低的约70%可归因于食物摄入量减少。部分生长抑制可能是由于支链氨基酸分解代谢增加，限制了缬氨酸和异亮氨酸用于生长的可用性。
