Chaudhary K D, Carrier-Malhotra L, Murthy M R
Neurochem Res. 1982 Jan;7(1):67-77. doi: 10.1007/BF00965070.
Transfer RNAs were isolated from rat and calf brains and their nucleosides were analysed by tritium derivative technique. Qualitative changes in the minor nucleoside components were compared on the fluorograms which showed differences in the intensities of spots. Cerebellar and cortical tRNAs were also compared, but revealed no significant quantitative differences in their methylated constituants despite 60% higher methyltransferase activity observed in cerebellum compared to cerebral cortex. An overall similarity was noticed between the relative proportions of the major and minor nucleosides of tRNAs derived from rat or calf brain, expressed as mol%. Brain tRNA was also analysed by two-dimensional polyacrylamide gel electrophoresis which showed qualitative and quantitative changes during postnatal development.
从大鼠和小牛大脑中分离出转运核糖核酸（tRNA），并通过氚衍生物技术分析其核苷。在荧光图谱上比较了次要核苷成分的定性变化，图谱显示斑点强度存在差异。还比较了小脑和皮质的tRNA，但尽管观察到小脑的甲基转移酶活性比大脑皮质高60%，但其甲基化成分在数量上没有显著差异。发现大鼠或小牛大脑来源的tRNA中主要和次要核苷的相对比例（以摩尔百分比表示）总体相似。还通过二维聚丙烯酰胺凝胶电泳分析了脑tRNA，结果显示其在出生后发育过程中存在定性和定量变化。
