几种哺乳动物tRNA在酰化二羟基硼酸纤维素和Aminex A - 28上的色谱行为

Chromatographic behavior of several mammalian tRNAs on acylated dihydroxyl-borate cellulose and Aminex A-28.

作者信息

Roe B A, Stankiewicz A F, Chen C Y

出版信息

Nucleic Acids Res. 1977 Jul;4(7):2191-204. doi: 10.1093/nar/4.7.2191.

DOI:10.1093/nar/4.7.2191

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342558/

Abstract

Studies of the chromatographic behavior of mammalian tRNAs, from several sources, on acylated DBAE-cellulose indicate that species of tRNA Asn , tRNA Asp and tRNA His can be retained on this matrix, while species of tRNA Tyr, tRNA Asn and tRNA Asp are not retained. Treatment of total rat liver tRNA with cyanogen bromide and subsequent chromatography on Aminex A-28 columns demonstrated that these tRNA species might contain Q (or Q*) nucleoside. However, comparable studies of the tRNA isolated from Walker 256 rat mammary tumor tissue demonstrated that this tumor tRNA almost totally lacks the hypermodified nucleosides Q and Q*. In addition, we have found that at least the major species of rat liver tRNA Asn contains the Q nucleoside. These studies indicate that chromatography on the acylated DBAE-cellulose matrix, couple with the analytical ion-exchange chromatography of cyanogen bromide treated and untreated amino-acyl-tRNA can be a valuable technique for the determination of alterations in the Q (or Q*) nucleoside content of the tRNAs isolated from normal and tumor tissues.

摘要

对来自多个来源的哺乳动物tRNA在酰化DBAE - 纤维素上的色谱行为研究表明，天冬酰胺tRNA、天冬氨酸tRNA和组氨酸tRNA的种类可以保留在这种基质上，而酪氨酸tRNA、天冬酰胺tRNA和天冬氨酸tRNA的种类则不能保留。用溴化氰处理大鼠肝脏总tRNA并随后在Aminex A - 28柱上进行色谱分析表明，这些tRNA种类可能含有Q（或Q*）核苷。然而，对从Walker 256大鼠乳腺肿瘤组织中分离的tRNA进行的类似研究表明，这种肿瘤tRNA几乎完全缺乏超修饰核苷Q和Q*。此外，我们发现至少大鼠肝脏天冬酰胺tRNA的主要种类含有Q核苷。这些研究表明，在酰化DBAE - 纤维素基质上进行色谱分析，再结合对经溴化氰处理和未处理的氨酰 - tRNA进行分析性离子交换色谱分析，可能是一种用于确定从正常组织和肿瘤组织中分离的tRNA的Q（或Q*）核苷含量变化的有价值技术。

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本文引用的文献

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3-(3-amino-3-carboxy-n-propyl)uridine. The structure of the nucleoside in Escherichia coli transfer ribonucleic acid that reacts with phenoxyacetoxysuccinimide.3-(3-氨基-3-羧基正丙基)尿苷。大肠杆菌转移核糖核酸中与苯氧乙酰氧基琥珀酰亚胺反应的核苷结构。

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Biochem Biophys Res Commun. 1973 Nov 16;55(2):477-83. doi: 10.1016/0006-291x(73)91111-x.

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