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用辣根过氧化物酶进行顺行标记后猫孤束核中迷走神经终末的超微结构鉴定。

The ultrastructural identification of vagal terminals in the solitary nucleus of the cat after anterograde labelling with horseradish peroxidase.

作者信息

Gwyn D G, Wilkinson P H, Leslie R A

出版信息

Neurosci Lett. 1982 Feb 12;28(2):139-43. doi: 10.1016/0304-3940(82)90142-2.

DOI:10.1016/0304-3940(82)90142-2
PMID:7070701
Abstract

Horseradish peroxidase (HRP) was injected into the nodose ganglion of the cat in order to label the axon terminals of vagal afferent fibres in the solitary nucleus. HRP reaction product was seen in axons, the majority of which were unmyelinated. Axon terminals containing round clear vesicles and an occasional dense-core vesicle were also labelled. These terminals synapsed upon both large and small dendritic elements, the majority of which were less than 1.0 micrometers in diameter. The labeling method appears to affect adversely the normal ultrastructural characteristics of terminals or their postsynaptic relationships and in these respects is superior to electron microscopic degeneration methods.

摘要

将辣根过氧化物酶(HRP)注入猫的结状神经节,以标记孤束核中迷走神经传入纤维的轴突终末。在轴突中可见HRP反应产物,其中大多数为无髓鞘的。含有圆形清亮小泡和偶尔的致密核心小泡的轴突终末也被标记。这些终末与大、小树突成分形成突触,其中大多数直径小于1.0微米。这种标记方法似乎对终末的正常超微结构特征或其突触后关系有不利影响,在这些方面优于电子显微镜下的变性方法。

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The ultrastructural identification of vagal terminals in the solitary nucleus of the cat after anterograde labelling with horseradish peroxidase.用辣根过氧化物酶进行顺行标记后猫孤束核中迷走神经终末的超微结构鉴定。
Neurosci Lett. 1982 Feb 12;28(2):139-43. doi: 10.1016/0304-3940(82)90142-2.
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