Teissie J, Knutson V P, Tsong T Y, Lane M D
Science. 1982 Apr 30;216(4545):537-8. doi: 10.1126/science.7071601.
Swiss mouse 3T3-C2 fibroblasts, grown to confluence in monolayer culture, are shown to fuse when exposed to electric fields. Exposure to five repetitive electric pulses of about 1 kilovolt per centimeter with a duration of 50 microseconds caused approximately 20 percent of the cells to become fused (multinucleate) when 1 millimolar magnesium was present in the medium. The effects of minimum thresholds of field strength, pulse duration, and number of pulses were determined. Cell disruption was observed when the electric field exceeded 2.0 kilovolts per centimeter or the pulse was of longer duration than 120 microseconds.
瑞士小鼠3T3 - C2成纤维细胞在单层培养中生长至汇合后，当暴露于电场时会发生融合。当培养基中存在1毫摩尔镁时，暴露于五个重复的电场脉冲（约每厘米1千伏，持续时间50微秒）会导致约20%的细胞发生融合（多核化）。确定了场强、脉冲持续时间和脉冲数的最小阈值效应。当电场超过每厘米2.0千伏或脉冲持续时间超过120微秒时，会观察到细胞破裂。
