Drynov I D, Melik-Andreasian G G, Puchkova N I, Skorikova A S, Uryvaev L V
Vopr Virusol. 1982 Jan-Feb(1):38-41.
Chronic infection with tick-borne encephalitis virus (Sophyin strain) has been established in continuous lines of human lymphocytes (of T- and B-origin). In 3 lines under study, the cultured virus at the level of the 15th passage had a titre of 7.22-8.02 lg LD50ml, the virus-specific antigen was determined in the cytoplasm of 80% of T-lymphocytes (line 1387). The method of RNA-DNA hybridization demonstrated the presence of virus-specific sequences in DNA preparations from infected cells in amounts not exceeding 1 copy of virus genome per 10-25 cells.
蜱传脑炎病毒（索菲因株）的慢性感染已在人淋巴细胞（T 细胞和 B 细胞来源）的连续系中建立。在研究的 3 个细胞系中，第 15 代培养病毒的滴度为 7.22 - 8.02 lg LD50/ml，在 80%的 T 淋巴细胞（1387 细胞系）细胞质中检测到病毒特异性抗原。RNA - DNA 杂交方法表明，感染细胞的 DNA 制剂中存在病毒特异性序列，其含量不超过每 10 - 25 个细胞 1 个病毒基因组拷贝。
