Iijima T, Wasano T
Am J Anat. 1978 Sep;153(1):171-6. doi: 10.1002/aja.1001530112.
The formaldehyde-induced fluorescence technique and the argentaffin reaction with ultra-thin sections were applied to the vessels of carp to demonstrate the presence of a catecholamine. After formaldehyde gas treatment for 30 minutes at 50 degrees C, the venous endothelia emitted a greenish-yellow fluorescence suggesting the presence of a primary catecholamine. In the endothelial cells of veins, the specific granules, consisting predominantly of spherical granules with a diameter of about 0.3 micrometer, showed heavily to moderately positive argentaffin reaction. Granules also occurred in the endothelial cells of arteries. However, these granules stained weakly or not at all with ammoniacal silver solution. It is concluded that the specific endothelial granules in veins of carp contain a primary catecholamine.
运用甲醛诱导荧光技术以及对超薄切片进行亲银反应，来检测鲤鱼血管中儿茶酚胺的存在情况。在50摄氏度下用甲醛气体处理30分钟后，静脉内皮发出黄绿色荧光，表明存在一种初级儿茶酚胺。在静脉的内皮细胞中，特定颗粒主要由直径约0.3微米的球形颗粒组成，呈现重度至中度的亲银反应。动脉内皮细胞中也有颗粒。然而，这些颗粒用氨性银溶液染色较弱或根本不染色。得出的结论是，鲤鱼静脉中的特定内皮颗粒含有一种初级儿茶酚胺。
