McAdaragh J P, Eustis S L, Nelson D T, Stotz I, Kenefick K
Am J Vet Res. 1982 Apr;43(4):693-6.
Four 6-week-old conventional pups were inoculated with a parvovirus (PV) isolated from the feces of a dog with naturally occurring enteritis. Blood for hematologic studies, virus isolation (VI), and antibody titration and feces for VI and negative-contrast electron microscopy were collected on day 0 and daily until necropsy. Beginning at postinoculation day 2, necropsies were done and specimens were collected for immunofluorescence, VI, and light microscopic examination. The PV infection was confirmed by VI, immunofluorescence, electron microscopy, and seroconversion. Clinical illness was not observed in inoculated pups, although mild intestinal lesions similar to those of naturally occurring PV enteritis were found. The failure to elicit severe disease in conventional pups indicates that one or more factors, such as intercurrent enteric or systemic infections, immune status, age, nutrition, virulence of virus, dose of infectious virus, and route of inoculation influence the clinical and pathologic manifestations of PV infection.
将4只6周龄的普通幼犬接种从一只患有自然发生性肠炎的犬粪便中分离出的细小病毒（PV）。在第0天采集用于血液学研究、病毒分离（VI）和抗体滴定的血液以及用于VI和负染色电子显微镜检查的粪便，之后每天采集直至尸检。从接种后第2天开始进行尸检，并采集标本用于免疫荧光、VI和光学显微镜检查。通过VI、免疫荧光、电子显微镜检查和血清转化确认了PV感染。尽管在接种的幼犬中发现了与自然发生的PV肠炎相似的轻度肠道病变，但未观察到临床疾病。在普通幼犬中未能引发严重疾病表明，一种或多种因素，如并发的肠道或全身感染、免疫状态、年龄、营养、病毒毒力、感染性病毒剂量和接种途径，会影响PV感染的临床和病理表现。
