Doyle M P, Roman D J
Appl Environ Microbiol. 1982 Mar;43(3):561-5. doi: 10.1128/aem.43.3.561-565.1982.
Studies were done to provide more comprehensive information on the response of Campylobacter jejuni and nalidixic acid-resistant, thermophilic Campylobacter (NARTC) to sodium chloride at 4, 25, and 42 degrees C. Three strains of C. jejuni were studies, and all could grow at 42 degrees C in the presence of 1.5% NaCl, but not 2.0% NaCl. At the same temperature, NARTC could grow in 2.0% NaCl and was substantially more tolerant to 2.5 and 4.5% NaCl than was C. jejuni. Both C. jejuni and NARTC grew poorly in the absence of added NaCl and grew best in the presence of 0.5% NaCl at 42 degrees C. At 25 degrees C, NaCl concentrations of 1.0 to 2.5% were protective to NARTC, but the same concentrations of salt generally enhanced the rate of death of C. jejuni. At 4 degrees C, both C. jejuni and NARTC were sensitive to 1.0% or more NaCl; however, the rate of death at this temperature was substantially less than that which occurred at 25 degrees C. A 3 log10 decrease of cells occurred in 4.5% NaCl after 1.2 to 2.1 days at 25 degrees C, and a similar reduction in cells took approximately 2 weeks at the same salt concentration and 4 degrees C. Although C. jejuni grows best in the presence of 0.5% NaCl, the presence of NaCl at concentrations as low as 1.0% may retard growth or increase rate of death; hence, it is advisable that growth media used for recovering or enumerating this organism contain 0.5% NaCl, but not 1.0% or more NaCl.
开展了多项研究，以提供关于空肠弯曲菌以及耐萘啶酸嗜热弯曲菌（NARTC）在4℃、25℃和42℃下对氯化钠反应的更全面信息。研究了三株空肠弯曲菌，所有菌株在42℃、1.5%氯化钠存在的情况下都能生长，但在2.0%氯化钠存在时则不能生长。在相同温度下，NARTC能在2.0%氯化钠中生长，并且比空肠弯曲菌对2.5%和4.5%氯化钠的耐受性要强得多。空肠弯曲菌和NARTC在未添加氯化钠时生长都很差，在42℃、0.5%氯化钠存在时生长最佳。在25℃时，1.0%至2.5%的氯化钠浓度对NARTC有保护作用，但相同浓度的盐通常会加快空肠弯曲菌的死亡速度。在4℃时，空肠弯曲菌和NARTC对1.0%或更高浓度的氯化钠都敏感；然而，在此温度下的死亡速度明显低于25℃时的死亡速度。在25℃下，4.5%氯化钠中细胞数量在1.2至2.1天后减少3个对数级，在相同盐浓度和4℃下，细胞数量减少类似幅度约需2周时间。尽管空肠弯曲菌在0.5%氯化钠存在时生长最佳，但低至1.0%浓度的氯化钠可能会阻碍其生长或加快死亡速度；因此，用于复苏或计数该菌的生长培养基宜含有0.5%氯化钠，而不是1.0%或更高浓度氯化钠。
