Pokhrel Diksha, Thames Hudson T, Zhang Li, Dinh Thu, Schilling M Wes, White Shecoya, Ramachandran Reshma, Sukumaran Anuraj T
Department of Poultry Science, Mississippi State University, Mississippi State, MS 39762, USA.
Tyson Foods, 2200 W. Don Tyson Parkway, Springdale, AR 72762, USA.
Foods. 2023 Sep 2;12(17):3305. doi: 10.3390/foods12173305.
is one of the leading causes of acute diarrhea in the United States. Despite being a microaerophilic pathogen, continues to endure within the domain of food production, especially in poultry processing. Recent research on aerotolerance indicates that close monitoring of this pathogen is necessary. A total of 40 isolates previously obtained from commercial broiler processing plants were analyzed for aerotolerance and genetic diversity. In addition, the effect of aerotolerance and storage time (days) on the survival of on broiler drumsticks at refrigeration (4 °C) and freezing conditions (-20 °C) was also evaluated. Out of 40 isolates, 25 (62.5%) were aero-sensitive (AS), 10 (25%) were intermediately aerotolerant (IAT), and 5 (12.5%) were hyper aerotolerant (HAT). The isolates belonged to four clonal complexes (CCs) and six sequence types, with the majority of isolates assigned to the CC-353 clonal complex. counts were reduced by 0.40 log CFU/g after 7 days at 4 °C and by 1.50 log CFU/g after 14 days at -20 °C, respectively, irrespective of aerotolerance ( < 0.001). At both refrigeration ( < 0.013) and freezing ( < 0.001), HAT showed greater reductions as compared to AS and IAT. These findings suggest that both refrigeration and freezing reduce counts.
是美国急性腹泻的主要原因之一。尽管它是一种微需氧病原体，但仍在食品生产领域持续存在，尤其是在禽类加工过程中。最近关于耐氧性的研究表明，对这种病原体进行密切监测是必要的。对先前从商业肉鸡加工厂获得的40株分离株进行了耐氧性和遗传多样性分析。此外，还评估了耐氧性和储存时间（天）对该菌在冷藏（4℃）和冷冻条件（-20℃）下在肉鸡鸡腿上存活的影响。在40株分离株中，25株（62.5%）对氧气敏感（AS），10株（25%）具有中等耐氧性（IAT），5株（12.5%）具有高耐氧性（HAT）。这些分离株属于四个克隆复合体（CCs）和六个序列类型，大多数分离株属于CC-353克隆复合体。无论耐氧性如何，在4℃下7天后菌数分别减少0.40 log CFU/g，在-20℃下14天后减少1.50 log CFU/g（P<0.001）。在冷藏（P<0.013）和冷冻（P<0.001）条件下，与AS和IAT相比，HAT的菌数减少幅度更大。这些发现表明，冷藏和冷冻都会减少菌数。
