人神经母细胞瘤和神经节神经母细胞瘤细胞系的建立与鉴定

Morrison L J, Cochran A J, Gibson A A, Willoughby M L, More I A

Br J Cancer. 1982 Apr;45(4):531-42. doi: 10.1038/bjc.1982.89.

The establishment in culture and characterization of 4 human neuroblastoma (NB) cell lines and 1 human ganglioneuroblastoma cell line are described. Each cell line fulfilled at least 2 of 4 criteria for malignant or transformed cells: viz., subcultured more than 70 times, high saturation density with absence of contact inhibition, population-doubling time within a range of 10-40 h, and tumour formation in nu/nu mice. Each cell line also fulfilled at least 2 of 3 criteria for neuroblastoma cells: viz., humoral and cell-mediated immune reactivity toward NB-associated cell-surface antigen, intracellular storage and extra-cellular secretion of catecholamines, and characteristic neuroblast and ganglion-cell morphology.

本文描述了4种人神经母细胞瘤（NB）细胞系和1种人神经节神经母细胞瘤细胞系的培养建立及特性鉴定。每个细胞系均满足恶性或转化细胞的4项标准中的至少2项，即：传代培养超过70次、高饱和密度且无接触抑制、群体倍增时间在10 - 40小时范围内以及在裸鼠体内形成肿瘤。每个细胞系还满足神经母细胞瘤细胞的3项标准中的至少2项，即：对NB相关细胞表面抗原的体液和细胞介导免疫反应性、儿茶酚胺的细胞内储存和细胞外分泌以及特征性的神经母细胞和神经节细胞形态。