Reisler E, Cheung P, Oriol-Audit C, Lake J A
Biochemistry. 1982 Feb 16;21(4):701-7. doi: 10.1021/bi00533a018.
Addition of KCl to a solution of synthetic myosin minifilaments in 10 mM citrate-Tris buffer (pH 8.0) induces the growth of filaments. These filaments, at pH 8.0, resemble in their morphological and hydrodynamic properties the synthetic filaments described by Josephs and Harrington [Josephs, R., & Harrington. W. F. (1966) Biochemistry 5, 3474--3487]. The rate of filament growth depends critically on the KCl concentration in the solution. Low rates of filament formation are noted in the presence of both low (below 80 mM KCl) and high (above 0.15 M KCl) salt concentrations, whereas at the intermediate KCl concentrations the filaments are formed at a fast rate. The formation of filaments from minifilaments is a reversible process, and under moderate salt concentrations, these two polymeric systems appear to exist in a dynamic equilibrium. Small amounts of minifilaments can induce rapid polymerization of dissociated myosin; i.e., they can act as a seeding material. These and other observations are discussed in terms of a direct route for filament formation from myosin minifilaments.
向10 mM柠檬酸盐 - Tris缓冲液（pH 8.0）中的合成肌球蛋白微丝溶液中添加氯化钾会诱导丝状物生长。这些在pH 8.0的丝状物，在形态和流体动力学性质上类似于约瑟夫斯和哈林顿所描述的合成丝状物[约瑟夫斯，R.，& 哈林顿，W. F.（1966年）《生物化学》5，3474 - 3487]。丝状物的生长速率严重依赖于溶液中的氯化钾浓度。在低盐浓度（低于80 mM氯化钾）和高盐浓度（高于0.15 M氯化钾）下，丝状物形成速率都较低，而在中等氯化钾浓度下，丝状物以快速形成。从微丝形成丝状物是一个可逆过程，并且在适度盐浓度下，这两个聚合物体系似乎处于动态平衡。少量微丝可诱导解离的肌球蛋白快速聚合；也就是说，它们可作为种子材料。根据从肌球蛋白微丝形成丝状物的直接途径对这些及其他观察结果进行了讨论。
