Franck P F, Roelofsen B, Op den Kamp J A
Biochim Biophys Acta. 1982 Apr 23;687(1):105-8. doi: 10.1016/0005-2736(82)90176-6.
Treatment of human erythrocytes with tetrathionate or diamide resulted in extensive crosslinking of membranous and cytoskeletal proteins. Such treatment was followed by an incubation with phosphatidylcholine specific exchange protein to investigate the rate and extent of exchange of phosphatidylcholine between the erythrocytes and 14C-labeled phosphatidylcholine containing microsomal membranes or vesicles. Exchange profiles showed that the exchange of phosphatidylcholine is facilitated in treated cells when compared to control erythrocytes and, more importantly, that all of the phosphatidylcholine is exchangeable after protein crosslinking whereas in control cells only the phosphatidylcholine pool located in the outer layer of the membrane is exchangeable. These observations demonstrate that crosslinking of cytoskeletal and membraneous proteins enhances the rate of transbilayer movement of phosphatidylcholine considerably.
用连四硫酸盐或二酰胺处理人红细胞会导致膜蛋白和细胞骨架蛋白广泛交联。随后将其与磷脂酰胆碱特异性交换蛋白一起孵育，以研究红细胞与含14C标记磷脂酰胆碱的微粒体膜或囊泡之间磷脂酰胆碱的交换速率和程度。交换图谱显示，与对照红细胞相比，经处理的细胞中磷脂酰胆碱的交换更容易，更重要的是，蛋白质交联后所有的磷脂酰胆碱都是可交换的，而在对照细胞中只有位于膜外层的磷脂酰胆碱池是可交换的。这些观察结果表明，细胞骨架蛋白和膜蛋白的交联显著提高了磷脂酰胆碱跨双层运动的速率。
