Scott S, Pendlebury S A, Green C
Biochem J. 1984 Nov 15;224(1):285-90. doi: 10.1042/bj2240285.
The aminophospholipids of microvesicles released from human erythrocytes on storage or prepared from erythrocyte ghosts by shearing under pressure are susceptible to the action of 2,4,6-trinitrobenzenesulphonic acid. The aminophospholipids of the former vesicles are also susceptible to attack by phospholipase A2. Under the same conditions, the aminophospholipids of erythrocytes undergo little reaction. This suggests that the phospholipids in microvesicle membranes are more randomly distributed than those in erythrocyte membranes. Measurements have also been made of the ability of filipin to react with the cholesterol of sealed and unsealed erythrocyte ghosts and of microvesicles prepared from them. From the initial rates of reaction, it was concluded that there is no preferential transfer of cholesterol molecules from one side of the bilayer to the other during the formation of the microvesicles.
储存时从人红细胞释放的微泡中的氨基磷脂，或通过压力剪切从红细胞影体制备的微泡中的氨基磷脂，易受2,4,6 - 三硝基苯磺酸的作用。前一种微泡中的氨基磷脂也易受磷脂酶A2的攻击。在相同条件下，红细胞中的氨基磷脂反应很少。这表明微泡膜中的磷脂比红细胞膜中的磷脂分布更随机。还测定了制霉菌素与密封和未密封的红细胞影体以及由它们制备的微泡中的胆固醇反应的能力。从初始反应速率得出结论，在微泡形成过程中，胆固醇分子没有从双层的一侧优先转移到另一侧。
