Alam S S, Layman D L
Biochim Biophys Acta. 1982 Mar 12;710(3):306-13. doi: 10.1016/0005-2760(82)90113-8.
Confluent cultures of human skin fibroblasts were exposed to medium containing high levels of low density lipoproteins (LDL-cholesterol equivalent to 400 micrograms per ml) and 0 or 2% dimethyl sulfoxide (DMSO). The uptake and accumulation of cellular cholesterol from LDL were reduced significantly (30%) in the DMSO-treated cells as compared to the controls. The reduction in cellular sterol was due almost exclusively to a significant decrease (50%) in cholesterol ester accumulation. Incubation of cells with 125I-labelled LDL showed clearly that DMSO did not act by increasing the secretion of cholesterol from the cell, but rather by significantly decreasing the binding, internalization and degradation of exogenous LDL. De novo synthesis of cholesterol from [14C]acetate was measured and found to correlate inversely with cellular sterol levels in either control or DMSO-treated cells.
将人皮肤成纤维细胞的融合培养物暴露于含有高水平低密度脂蛋白（相当于每毫升400微克的低密度脂蛋白胆固醇）和0%或2%二甲基亚砜（DMSO）的培养基中。与对照相比，经DMSO处理的细胞中来自低密度脂蛋白的细胞胆固醇摄取和积累显著降低（30%）。细胞固醇的减少几乎完全是由于胆固醇酯积累显著减少（50%）。用125I标记的低密度脂蛋白孵育细胞清楚地表明，DMSO的作用不是通过增加胆固醇从细胞中的分泌，而是通过显著降低外源性低密度脂蛋白的结合、内化和降解。测定了由[14C]乙酸酯从头合成胆固醇的量，发现其与对照或经DMSO处理的细胞中的细胞固醇水平呈负相关。
