Bell F P, Hubert E V
Lipids. 1982 Dec;17(12):900-4. doi: 10.1007/BF02534585.
The incorporation of [14C]mevalonate and [14C]acetate into squalene by rat liver slices was increased over 7-fold by the presence of 5% dimethylsulfoxide (DMSO) in the incubation medium. The stimulation of squalene synthesis was dose-related over the concentration range of 1-5% DMSO and did not affect the incorporation of [14C]mevalonate into the C27-sterol fraction (cholesterol) but did increase (about 50%) incorporation into C30-sterol (lanosterol) at a level of 5% DMSO. The stimulation of squalene synthesis was observed under both anaerobic (N2 atmosphere) and aerobic (ambient air or 95% O2/5% CO2) conditions and may represent a direct effect of DMSO on squalene synthetase. At a level of 5%, DMSO also stimulated 7-fold the incorporation of [14C]acetate into triglycerides by liver slices; this occurred without changes in incorporation into the phospholipid or free fatty acid fractions. The disproportionate increase in lipid labeling from [14C]acetate suggests that the effects of DMSO are not simply a matter of increasing [14C]acetate entry into the tissue.
在孵育培养基中加入5%的二甲基亚砜（DMSO）后，大鼠肝切片将[14C]甲羟戊酸和[14C]乙酸掺入角鲨烯的量增加了7倍多。在1-5% DMSO的浓度范围内，角鲨烯合成的刺激作用与剂量相关，且不影响[14C]甲羟戊酸掺入C27-甾醇部分（胆固醇），但在5% DMSO水平下，确实增加了（约50%）其掺入C30-甾醇（羊毛甾醇）的量。在厌氧（N2气氛）和好氧（环境空气或95% O2/5% CO2）条件下均观察到角鲨烯合成受到刺激，这可能代表DMSO对角鲨烯合酶的直接作用。在5%的水平下，DMSO还使肝切片将[14C]乙酸掺入甘油三酯的量增加了7倍；这种情况发生时，掺入磷脂或游离脂肪酸部分的量没有变化。[14C]乙酸脂质标记的不成比例增加表明，DMSO的作用不仅仅是增加[14C]乙酸进入组织的量。
