Kachurin A M, Fomichev V N
Biofizika. 1982 Mar-Apr;27(2):212-7.
Geometry of the complexes between horse radish peroxidase and aromatic substrates was studied by means of new technique of precision measurement of NMR shifts. Weak dipolar shifts of hydroquinone and parametoxiphenole PMR in the presence of high spin peroxidase were recorded. Polar and azimuth angles in coordinate axes of peroxidase hem for the binding site on the enzyme of the aromatic part of the substrate molecule were estimated for the first time.
利用核磁共振位移精确测量新技术研究了辣根过氧化物酶与芳香底物之间复合物的几何结构。记录了在高自旋过氧化物酶存在下对苯二酚和对甲氧基苯酚的弱偶极位移。首次估计了底物分子芳香部分在过氧化物酶血红素坐标轴上结合位点的极角和方位角。
