[Enzyme inactivation in the course of the reaction. Kinetic description and discrimination of mechanisms].

The kinetic regularities of the enzymatic reaction involving substrate depletion and enzyme inactivation were studied. Various inactivation mechanism including monomolecular inactivation of the free enzyme or the enzyme--substrate complex and bimolecular inactivation with the substrate and reaction product were investigated. The possibility of discrimination of these mechanisms and determination of the kinetic parameters for these processes based on integral equations are discussed.