[Characterization of DNAse from rat brain].

Using gel filtration and ion exchange chromatography, a DNAse specific to a single-stranded DNA was isolated from the soluble fraction of rat brain nuclear proteins. The nuclear nuclease corresponds to alkaline DNAse obtained earlier from the intact brain. The enzyme is a Mg2+- or Mn2+-dependent exodeoxyribonuclease and hydrolyzes at the same rates the ss-DNAse from rat brain, calf thymus, E. coli and poly (dA). The major products of hydrolysis are nucleoside-5'-monophosphates. A possible role of the enzyme in reparation of neuronal DNA is discussed.