Kostiuchenko D A, Kolomiĭtseva G Ia, Kostiuchenko V I, Ptitsyn L R
Biokhimiia. 1982 Mar;47(3):497-501.
The release of DNAs from nuclear preparations isolated at different steps of the cell cycle of the synchronous culture of Physarum polycephalum and irradiated with UV-light for 5 min (lambda 253.7 A, i = 1.8 . 10(2) j . m-2 . min-1) into 1% Na-DS - 1.5 M NaCl solution was studied. The value obtained correlated well with the degree of binding of interacting proteins with DNA in chromatin and was identical for all the preparations tested with the only exception of the nuclei isolated at the end of the S-phase, when a slight but significant (15 +/- 10%) increase in DNA release was observed. The results obtained are discussed in terms of structural transitions and function of chromatin during the cell cycle of the myxomycete Physarum polycephalum.
研究了在多头绒泡菌同步培养的细胞周期不同阶段分离得到的细胞核制剂，经紫外线（波长253.7 Å，辐照度为1.8×10² J·m⁻²·min⁻¹）照射5分钟后，DNA释放到1%十二烷基硫酸钠-1.5 M氯化钠溶液中的情况。所得到的值与染色质中相互作用蛋白与DNA的结合程度密切相关，并且除了在S期结束时分离得到的细胞核外，所有测试制剂的结果均相同，在S期结束时观察到DNA释放略有但显著（15±10%）增加。从多头绒泡菌细胞周期中染色质的结构转变和功能方面对所得结果进行了讨论。
