Murphy R F, Daban J R, Cantor C R
Cytometry. 1981 Jul;2(1):26-30. doi: 10.1002/cyto.990020106.
The nuclear cycle kinetics of Physarum polycephalum plasmodia were examined using flow cytofluorometry. The dyes Hoechst 33342 and propidium iodide were used to stain the DNA of isolated nuclei. In asynchronously growing microplasmodia. S phase consists of 13--15% of the nuclear division cycle time. Nuclei isolated from individual macroplasmodia, which have previously been demonstrated to divide in synchrony, were shown to be less synchronized during late S phase than during mitosis. The results obtained demonstrate the feasibility of flow cytometric measurement of the properties of nuclei isolated from a single cell.
利用流式细胞荧光测定法检测了多头绒泡菌原质团的核循环动力学。使用Hoechst 33342和碘化丙啶染料对分离出的细胞核DNA进行染色。在异步生长的微原质团中，S期占核分裂周期时间的13%-15%。从先前已证明能同步分裂的单个大原质团中分离出的细胞核，在S期后期的同步性低于有丝分裂期。所获得的结果证明了通过流式细胞术测量从单个细胞中分离出的细胞核特性的可行性。
