尿多巴胺、去甲肾上腺素和肾上腺素的同步荧光测定法与液相色谱-电化学检测法的比较
Simultaneous fluorometry of urinary dopamine, norepinephrine, and epinephrine compared with liquid chromatography with electrochemical detection.
作者信息
Oka K, Sekiya M, Osada H, Fujita K, Kato T, Nagatsu T
出版信息
Clin Chem. 1982 Apr;28(4 Pt 1):646-9.
A simple fluorometric method is described for simultaneous assay of urinary norepinephrine, epinephrine, and dopamine. The catecholamines are separated by boric acid gel chromatography and subsequently determined by use of a modified trihydroxyindole reaction. By the separation with boric acid gel, dopa can be eliminated, which interferes with dopamine measurement; consequently, the reaction is specific for catecholamines. This simple, rapid method was applied to urine specimens from normal adults. Values for urinary dopamine, norepinephrine, and epinephrine agreed with those obtained by "high-performance" liquid chromatography with electrochemical detection.
本文描述了一种用于同时测定尿中去甲肾上腺素、肾上腺素和多巴胺的简单荧光法。儿茶酚胺通过硼酸凝胶色谱法分离，随后使用改良的三羟基吲哚反应进行测定。通过硼酸凝胶分离，可以消除干扰多巴胺测量的多巴；因此，该反应对儿茶酚胺具有特异性。这种简单、快速的方法应用于正常成年人的尿液标本。尿多巴胺、去甲肾上腺素和肾上腺素的值与通过高效液相色谱-电化学检测获得的值一致。
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