Abmayr W, Giaretti W, Gais P, Dörmer P
Cytometry. 1982 Mar;2(5):316-26. doi: 10.1002/cyto.990020509.
Images of exponentially growing mouse L fibroblasts were used to test whether G1, S, and G2 cells could be discriminated by means of densitometric, geometric, textural and chromatin features. The cells were preincubated with FUdR and labeled with 3H-TdR. The definition of G1, S, and G2 was based on autoradiography used to define labeled S cells, and DNA content used to define unlabeled 2c (G1) and 4c (G2) cells. The methanol-fixed Feulgen-stained nuclei were scanned with a TV Plumbicon camera equipped with an array-processor system. Thirty-one nuclear features were calculated and stored together with the precise coordinates of each cell for later relocation and correlation with the autoradiographic data. The features were geometric, densitometric, textural and chromatin parameters of the Feulgen stained images. Feature evaluation and supervised learning were performed. When DNA content was excluded 14 textural and chromatin features were selected. By using these a correct classification of about 80% was achieved. Inclusion of DNA content and its correlates caused degeneration of performance, yielding 75% correct classification. DNA content alone gave 69% correct classification.
利用指数生长的小鼠L成纤维细胞的图像，来测试G1期、S期和G2期细胞是否可以通过光密度、几何、纹理和染色质特征进行区分。细胞先用氟尿苷预孵育，然后用³H-胸腺嘧啶核苷标记。G1期、S期和G2期的定义基于用于定义标记S期细胞的放射自显影，以及用于定义未标记的2c（G1期）和4c（G2期）细胞的DNA含量。用配备了阵列处理器系统的电视氧化铅摄像管对甲醇固定、福尔根染色的细胞核进行扫描。计算了31个核特征，并将其与每个细胞的精确坐标一起存储，以便后续重新定位并与放射自显影数据进行关联。这些特征是福尔根染色图像的几何、光密度、纹理和染色质参数。进行了特征评估和监督学习。当排除DNA含量时，选择了14个纹理和染色质特征。通过使用这些特征，实现了约80%的正确分类。纳入DNA含量及其相关因素导致性能下降，正确分类率为75%。仅DNA含量的正确分类率为69%。
