Ivey K J, Tarnawski A, Krause W J, Stachura J, Sherman D, Burks M
Dig Dis Sci. 1982 May;27(5):394-400. doi: 10.1007/BF01295646.
The effect on parietal cells of glucagon given prior to pentagastrin is unknown. Fifteen healthy volunteers were studied during constant intravenous infusion of pentagastrin (2 micrograms/kg body weight/hr) and during pentagastrin infusion initiated 20 min after intravenous injection of 2 mg glucagon. Three types of studies were performed: Gastric mucosal biopsies were obtained with a Quinton instrument. Electron micrographs of 320 parietal cells were analyzed by the Loud quantitative method, and intragastric pH (pH probe), or gastric potential difference (PD) were recorded continuously. Pentagastrin infusion produced a significant increase in canalicular and simultaneous reduction of tubulovesicular membrane area of parietal cells. Glucagon pretreatment did not inhibit canalicular and tubulovesicular membrane reaction to pentagastrin; unexpectedly this reaction was significantly greater when compared to that after pentagastrin alone. Initiation of pentagastrin infusion in subjects pretreated with glucagon produced a greater absolute value drop in gastric PD (23 +/- 2 mV) and an earlier drop in intragastric pH (3 min) than in subjects receiving pentagastrin infusion alone (13 +/- 1 mV and 10 min, respectively). In conclusion, at the doses studied, glucagon pretreatment increases parietal cell canalicular reaction to pentagastrin.
在五肽胃泌素之前给予胰高血糖素对壁细胞的影响尚不清楚。对15名健康志愿者进行了研究，在持续静脉输注五肽胃泌素（2微克/千克体重/小时）期间以及在静脉注射2毫克胰高血糖素20分钟后开始输注五肽胃泌素期间进行研究。进行了三种类型的研究：用昆顿器械获取胃黏膜活检组织。通过劳德定量法分析320个壁细胞的电子显微照片，并连续记录胃内pH值（pH探头）或胃电位差（PD）。输注五肽胃泌素使壁细胞的微管显著增加，同时使微管泡状膜面积减少。胰高血糖素预处理并未抑制壁细胞对五肽胃泌素的微管和微管泡状膜反应；出乎意料的是，与单独使用五肽胃泌素后的反应相比，这种反应明显更大。在接受胰高血糖素预处理的受试者中开始输注五肽胃泌素时，胃PD的绝对值下降幅度更大（23±2毫伏），胃内pH值下降更早（3分钟），而单独接受五肽胃泌素输注的受试者分别为13±1毫伏和10分钟。总之，在所研究的剂量下，胰高血糖素预处理会增加壁细胞对五肽胃泌素的微管反应。
