Brinkmann A, Leemborg I, Rommerts F, van der Molen H
Endocrinology. 1982 May;110(5):1834-6. doi: 10.1210/endo-110-5-1834.
We have investigated the effects of hCG and estradiol on the time course of the inhibition of C17-20-lyase and the depletion of the estradiol cytosol receptor in testicular tissue of male rats. In hCG-treated animals the first significant decrease in C17-20-lyase activity was observed 16 h after injection of the hormone. The C17-20-lyase activity decreased further during the following 56 hours. Estradiol-cytosol receptor levels were significantly decreased 7 hours after the hCG injection, and after a nadir at 16 hours increased to 130% of control values at 72 hours. In contrast, in the estradiol-treated animals the first significant inhibition of C17-20-lyase activity could be observed only after 24 h, while a 95% depletion of estradiol-cytosol receptor levels was observed already within 1 h after administration of the hormone. These results demonstrate that a rapid estradiol-induced depletion of the estradiol receptor as such does not necessarily result in a subsequent immediate inhibition of the C17-20-lyase. The rapid inhibition of this enzyme after hCG indicates, therefore, that other mechanisms are involved.
我们研究了人绒毛膜促性腺激素（hCG）和雌二醇对雄性大鼠睾丸组织中17 - 20碳链裂解酶抑制的时间进程以及雌二醇胞质受体耗竭的影响。在接受hCG治疗的动物中，注射激素16小时后观察到17 - 20碳链裂解酶活性首次出现显著下降。在接下来的56小时内，17 - 20碳链裂解酶活性进一步降低。hCG注射7小时后，雌二醇胞质受体水平显著下降，在16小时达到最低点后，在72小时时升至对照值的130%。相比之下，在接受雌二醇治疗的动物中，仅在24小时后才能观察到17 - 20碳链裂解酶活性的首次显著抑制，而在给予激素后1小时内，雌二醇胞质受体水平就已出现95%的耗竭。这些结果表明，雌二醇诱导的雌二醇受体快速耗竭本身并不一定会导致随后立即抑制17 - 20碳链裂解酶。因此，hCG注射后该酶的快速抑制表明还涉及其他机制。
