Melner M H, Abney T O
Endocrinology. 1980 Nov;107(5):1620-6. doi: 10.1210/endo-107-5-1620.
Recent studies have shown that hCG stimulates 17 beta-estradiol production in the testis, and a possible role for 17 beta-estradiol in hCG-induced desensitization of testosterone synthesis has been suggested. These studies were initiated to examine the testicular content of cytoplasmic estrogen receptor in relation to hCG-induced desensitization of testosterone production 24 h and 5 days after a sc injection of hCG. Twenty-four hours after the injection of 30, 300, and 3000 IU hCG, pronounced (82-88%) depletion of the cytoplasmic estrogen receptor was observed, while a 3-IU dose elicited a 41% depletion. A concomitant desensitization to hCG stimulation in vitro was observed after the injection of 30, 300, and 3000 IU hCG, as reflected by a lack of stimulation of testosterone production above that in matched controls. Testicular 17 beta-estradiol levels rose significantly after the injection of 300 or 3000 IU hCG. Five days after the injection of hCG, full replenishment of the cytoplasmic estrogen receptor had occurred in the 3- and 30-IU dose groups, while partial replenishment (22-56% depletion) had occurred with the 300- and 3000-IU dose groups. Only the 3000-IU dose group remained desensitized to hCG stimulation in vitro at this time point. Results indicated that occupancy by endogenous 17 beta-estradiol was not a factor, thus suggesting a true receptor depletion phenomenon. These results demonstrate that hCG-induced desensitization of testicular steroidogenesis is accompanied by depletion of the cytoplasmic estrogen receptor. Further, replenishment of the estrogen receptor at 5 days was accompanied by a return of the testicular response to hCG.
近期研究表明，人绒毛膜促性腺激素（hCG）可刺激睾丸中17β - 雌二醇的生成，并且有人提出17β - 雌二醇在hCG诱导的睾酮合成脱敏过程中可能发挥作用。开展这些研究是为了检测皮下注射hCG后24小时和5天时，细胞质雌激素受体的睾丸含量与hCG诱导的睾酮生成脱敏之间的关系。注射30、300和3000国际单位hCG后24小时，观察到细胞质雌激素受体明显（82 - 88%）耗竭，而3国际单位的剂量引起41%的耗竭。注射30、300和3000国际单位hCG后，在体外观察到对hCG刺激的同时脱敏，这表现为睾酮生成的刺激程度未超过匹配对照组。注射300或3000国际单位hCG后，睾丸17β - 雌二醇水平显著升高。注射hCG 5天后，3国际单位和30国际单位剂量组的细胞质雌激素受体已完全补充，而300国际单位和3000国际单位剂量组出现部分补充（22 - 56%的耗竭）。仅3000国际单位剂量组在此时点对体外hCG刺激仍处于脱敏状态。结果表明内源性17β - 雌二醇的占据并非一个因素，因此提示存在真正的受体耗竭现象。这些结果表明，hCG诱导的睾丸类固醇生成脱敏伴随着细胞质雌激素受体的耗竭。此外，5天时雌激素受体的补充伴随着睾丸对hCG反应的恢复。
