抗生物素蛋白的结晶及初步X射线研究。

Crystallization and preliminary X-ray investigation of avidin.

作者信息

Pinn E, Pähler A, Saenger W, Petsko G A, Green N M

出版信息

Eur J Biochem. 1982 Apr;123(3):545-6. doi: 10.1111/j.1432-1033.1982.tb06566.x.

DOI:10.1111/j.1432-1033.1982.tb06566.x

Abstract

Hen egg-white avidin was crystallized at pH 6 from solutions containing 8-9% (w/v) poly(ethyleneglycol) 6000 as precipitant. X-ray investigation showed that the crystals are orthorhombic with cell constants a = 7.47 nm, b = 8.00 nm, c = 4.31 nm and belong to space group P21212. They contain half of a tetrameric molecule in the asymmetric unit.

摘要

在pH值为6的条件下，以含有8-9%（重量/体积）聚乙二醇6000作为沉淀剂的溶液使蛋清抗生物素蛋白结晶。X射线研究表明，这些晶体为正交晶系，晶胞常数a = 7.47纳米，b = 8.00纳米，c = 4.31纳米，属于空间群P21212。它们在不对称单元中包含四聚体分子的一半。